ABSTRACT
The present study was carried on 50 Friesian female cows [3-5 years old] in El-Minya Province, Egypt. Serological investigation of cattle for brucellosis revealed that, 68, 56, 60, 48 and 56% of the tested cows were brucella reactant to the Buffered Acidified Plate Antigen Test [BAPAT], Rose Bengal Plate Test [RBPT], Serum Agglutination Test [SAT], Rivanol Test [RT] and Complement Fixation Test [CFT], respectively. Biochemical analysis of the tested cows sera showed that brucella reactant cows had a significant increase in serum Alanine Amino Transferase [ALT], Aspartate Amino Transferase [AST], Alkaline Phosphatase [AP], Urea and Creatinine levels. Significant increase of serum total proteins, globulin, lipids, cholesterol, triacylglycerol, free fatty acids and the high, low and very low density lipoproteins were also detected. On the other hand significant decrease in serum albumin, A/G ratio, phospholipids, calcium, inorganic phosphorus, magnesium, sodium and potassium levels were shown among the bruccella reactant Friesian cows compared to the non-reactant controls. It could be concluded that, in addition to the hazard of distributing brucella infection, brucella reactant cows under study suffered hepatic and renal dysfunction that altered their serum constitution, which in turn implies itself on the animal health and they should be eradicated from the producing cattle herd as soon as possible